Here, we have developed biomimetic nanocomposites containing chitosan, poly(vinyl alcohol) and nano-hydroxyapatite-zinc oxide as bone extracellular matrix for human osteoblastic cells and characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction. Scanning electron microscopy images revealed interconnected macroporous structures. Moreover, in this study, the problem related to fabricating a porous composite with good mechanical strength has been resolved by incorporating 5 wt% of nano-hydroxyapatite-zinc oxide into chitosan-poly(vinyl alcohol) matrix; the present composite showed high tensile strength (20.25 MPa) while maintaining appreciable porosity (65.25%). These values are similar to human cancellous bone. These nanocomposites also showed superior water uptake, antimicrobial and biodegradable properties than the previously reported results. Compatibility with human blood and pH was observed, indicating nontoxicity of these materials to the human body. Moreover, proliferation of osteoblastic MG-63 cells onto the nanocomposites was also observed without having any negative effect. © 2016 Elsevier Ltd. All rights reserved.

A BHOWMICK

T MITRA

A GNANAMANI

M DAS

P P KUNDU

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

Carbohydrate Polymers

The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite. Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites. Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains were established for ZnCMH I-III. pH and blood compatibility study revealed that ZnCMH I-III should be nontoxic to the human body. Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells was also established. Experimental findings suggest that addition of 5 wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity. Addition of 0.1 wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite. In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering. © 2017 Elsevier B.V.

International Journal of Biological Macromolecules

Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering

ZrO2 nanoparticle (NP) doped CTS-PVA-HAP composites (ZrCPH I-III) were developed and characterized by FT-IR and XRD studies to mimic human bone for bone tissue engineering applications. Interconnected porous structures of these composites were observed via SEM and the porosities were in the range of human cancellous bone. These composites also have good swelling abilities both in aqueous and SBF media. The addition of ZrO2 NPs into the CTS-PVA-HAP composites improved the tensile strength of ZrCPH I-III compared with previously reported CTS-PVA-HAP composites, and the maximum tensile strength was obtained with ZrCPH III (CTS:PVA:HAP-ZrO2 = 55:30:15 wt%), which had the highest ZrO2 content (0.3 wt%). The strongest antimicrobial effect was also observed for ZrCPH III, which had the maximum amount of nano-HAP-ZrO2. Cytocompatibility with human osteoblastic MG-63 cells was also established and the highest cell proliferation was observed with ZrCPH III. Thus, ZrCPH III should have the potential to be applied as a bone tissue engineering material. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2017.

New Journal of Chemistry

Mechanical and biological investigations of chitosan-polyvinyl alcohol based ZrO2 doped porous hybrid composites for bone tissue engineering applications

In this paper, we report a simple approach for the fabrication of bone-like composite materials (BCMs) by blending chitosan (CTS), a natural polysaccharide, nano-hydroxyapatite as bone mineral, and polymethylmethacrylate- co-polyhydroxyethylmethacrylate (PMMA-co-PHEMA) as bone cement. Fourier transform infrared spectroscopy and X-ray diffraction (XRD) studies were performed to determine the components of composites. Scanning electron microscopy study of composites revealed a well-dispersed flake-like structure with increasing content of PMMA-co-PHEMA. The water-uptake ability of the BCMs was also found to increase with increasing the PMMA-co-PHEMA content. Study of mechanical properties revealed that the Young's modulus and stiffness increased significantly after the addition of PMMA-co-PHEMA in BCMs compared with CTS-HAP blend. © 2013 Wiley Periodicals, Inc.

Advances in Polymer Technology

Multicomponent fabrication of bone-like composite materials using chitosan/pmma-co-phema/nano-hydroxyapatite

Journal of Nanobiotechnology

Nanohydroxyapatite-reinforced chitosan composite hydrogel for bone tissue repair in vitro and in vivo

Materials Science and Technology

Stability of chitosan/montmorillonite nanohybrid towards enzymatic degradation on grafting with poly(lactic acid)

Biomater. Sci.

The synergistic effect of a hybrid graphene oxide–chitosan system and biomimetic mineralization on osteoblast functions

Acta Biomaterialia

The interplay between nanostructured carbon-grafted chitosan scaffolds and protein adsorption on the cellular response of osteoblasts: Structure–function property relationship

Development of biomimetic nanocomposites as bone extracellular matrix for human osteoblastic cells

Journal	Data powered by TypesetCarbohydrate Polymers
Publisher	Data powered by TypesetElsevier Ltd
ISSN	0144-8617