Fibrinogen conjugated gold nanoparticles (fibrinogen-Au) and thrombin conjugated silver nanoparticles (thrombin-Ag) were synthesized by heating (90 °C) the proteins (50 μg protein/ml) with 1 mM AgNO3 or AuCl3. The resultant particles were harvested and examined by flow cytometry, scanning electron microscopy (SEM), transmission emission microscopy (TEM), optical microscopy and dynamic light scattering. SEM and TEM images revealed that the fibrinogen-Au and thrombin-Ag particles interacted. The emergent bio-nanoconjugate population could be controlled by addition of thrombin-Ag. The method may be exploited in parametrizing coagulation factors and other clinically important protein-protein interactions. © 2007 Federation of European Biochemical Societies.

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A K DASGUPTA

Fulltext

University of Calcutta (informally known as Calcutta University or CU) is a collegiate public state university located in Kolkata, West Bengal, India.&nbsp;Within India it is recognized as a &quot;Five-Star University&quot; and accredited &quot;A&quot; Grade by National Assessment and Accreditation Council.

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The University of Calcutta has secured the Fifth position among the Universities of India in the prestigious &quot;Indian University Ranking 2019&quot; list, released by the NIRF of the Ministry of Human Resource Development, Government of India.

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It was established on 24 January 1857, and was one of the first institutions in Asia to be established as a multidisciplinary and Western-style university. Its alumni and faculty include several heads of state, heads of government, social reformers, prominent artists, only academy award winner and Dirac medal winner in India, many Fellows of the Royal Society and five Nobel laureates as of 2019 which is highest in South Asia.

University Of Calcutta

FEBS Letters

Citrate synthase is a heat labile enzyme showing a loss of activity even in response to a modest shift of temperature (35°-45°C). Gold nanoparticle is shown to prevent the thermal aggregation of this enzyme. The chaperon like activity of the nanoparticle diminishes if the particle size is reduced from 40 nm to 20 nm keeping the atomic concentration of gold constant. This implies that the effect is not merely due to enhanced surface area offered by the nano-surfaces. The effect is reversible as the protein separated out from nanoparticles behaves similar to control. The observed coupling between chaperon activity of the nanoparticle and its cluster forming ability is illustrated in terms of a thermal cage model. Copyright © 2010 American Scientific Publishers All rights reserved.

Journal of Nanoscience and Nanotechnology

Size dependent chaperon properties of gold nanoparticles

Anti-glycation activity of gold nanoparticles (GNPs) has been reported for the first time. Nonenzymatic glycation of α-crystallin leads to formation of cataract, or opaque aggregate of proteins. In this article we report prevention of glycation of α-crystallin by conjugation with GNPs. Formation of advanced glycosylic end products is prevented even if a strong glycating agent such as fructose is used. In addition, the nanoconjugation can provide some important information on the structural distribution of this dynamic chaperone protein. Because GNPs are biocompatible, their reported anti-glycation activity may have ophthalmological implications. © 2009 Elsevier Inc. All rights reserved.

Journal	FEBS Letters
Publisher	-
ISSN	0014-5793