To confirm whether there exists any regional responses of the adrenocortical tissue to aldosterone activity in birds, an experiment was performed using anti-mineralocorticoids like spironolactone and captopril. These two were administered orally to pigeon continually for 7 days. As a result of this experimental manipulation, histological hypoactivity was found to be pronounced in the peripheral as well as in the central cortical cords. Thus the present report suggests bizonal synthesis of aldosterone in the pigeon adrenal cortex. Moreover, an increased level of glandular corticosterone along with identical alterations in sodium - potassium ratio of the serum were noticed in both the treated groups.