Header menu link for other important links
X
Carnosic acid, a natural diterpene, attenuates arsenic-induced hepatotoxicity via reducing oxidative stress, MAPK activation, and apoptotic cell death pathway
S DAS, S JOARDAR, P MANNA, T K DUA, N BHATTACHARJEE, R KHANRA, S BHOWMICK, J KALITA, , S RAYShow More
Published in Hindawi Limited
2018
PMID: 29854073
Volume: 2018
   
Abstract
The present studies have been executed to explore the protective mechanism of carnosic acid (CA) against NaAsO 2 -induced hepatic injury. CA exhibited a concentration dependent (1-4 μM) increase in cell viability against NaAsO 2 (12 μM) in murine hepatocytes. NaAsO 2 treatment significantly enhanced the ROS-mediated oxidative stress in the hepatic cells both in in vitro and in vivo systems. Significant activation of MAPK, NF-κB, p53, and intrinsic and extrinsic apoptotic signaling was observed in NaAsO 2 -exposed hepatic cells. CA could significantly counteract with redox stress and ROS-mediated signaling and thereby attenuated NaAsO 2 -mediated hepatotoxicity. NaAsO 2 (10 mg/kg) treatment caused significant increment in the As bioaccumulation, cytosolic ATP level, DNA fragmentation, and oxidation in the liver of experimental mice (n = 6). The serum biochemical and haematological parameters were significantly altered in the NaAsO 2 -exposed mice (n = 6). Simultaneous treatment with CA (10 and 20 mg/kg) could significantly reinstate the NaAsO 2 -mediated toxicological effects in the liver. Molecular docking and dynamics predicted the possible interaction patterns and the stability of interactions between CA and signal proteins. ADME prediction anticipated the drug-likeness characteristics of CA. Hence, there would be an option to employ CA as a new therapeutic agent against As-mediated toxic manifestations in future. © 2018 Sonjit Das et al.
About the journal
JournalOxidative Medicine and Cellular Longevity
PublisherHindawi Limited
ISSN1942-0900
Open AccessNo