The present study was aimed to establish the augmentative property of crude Pleurotus florida mushroom extract when taken with other established anti-diabetic drugs, i.e, metformin. Alloxan-induced diabetic mice were treated with metformin and metformin along with Pleurotus floridaextract to compare the hypoglycemic profile of these two groups of mice. Results showed a good decline in blood glucose levels in both acute and sub-acute studies in group of mice having combined treatment. Malondialdehyde concentration was also assayed to find out any protective property of this mushroom against late diabetic complications through inhibition of ROS (Reactive Oxygen Species)formation in the liver. © 2014 Sphinx Knowledge House. All Rights reserved.