To find the action of ethanolic extract of Aegle marmelos leaves (EAM) on spermatogenesis in albino rats 25 mg and 50 mg/kg body weight. EAM was administered orally for 30 days. Weight of primary and some accessory sex organs, sperm count and sperm motility and biochemical estimation of testicular protein and RNA were considered to study the effect of EAM on male reproductive function. EAM has showed significant decrease in the weight of sex organs, reduced sperm count, increased sperm motility and both protein and RNA contents of testis dose dependently. All these results indicted the anti-spermatogenic properties of Aegle marmelos in albino rats.