Therapeutic application of herbs against various pathophysiological conditions is well known and several herbs and herbal formulations have been mentioned in Indian Ayurvedic literature. PulmoFlex is a polyherbal formulation of ten standardised herbal extracts, manufactured by Indian Herbs, Saharanpur, India. This product is claimed to provide long lasting relief in allergic disorders through its immunomodulatory, mast cell stabilizing, antiallergic and bronchodilator actions. The present study was designed to explore the antihistaminic and antianaphylactic property of PulmoFlex in experimental animals. In in-vitro studies, PulmoFlex significantly inhibited compound 48/80 induced mast cell degranulation. PulmoFlex significantly protected histamine aerosol induced collapse in guineapig and compound 48/80 induced histamine release from guineapig chopped lung preparation. PulmoFlex also exhibited significant protection in passive foot anaphylaxis and passive cutaneous anaphylaxis in albino mice. Bovine serum albumin induced anaphylactic shock in male albino mice was also antagonised by PulmoFlex.